Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

THP-1 WCE ChIPseq

cell line

THP-1

cell type

monocytic cell

genotype

MLL-AF9

chip antibody

No antibody

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Protein-DNA complexes were immunoprecipitated from sonicated chromatin extract by the specific antibody. ChIP-seq DNA sample prep kit (Illumina, catalog number 1003473) according to the manufacturer’s protocol. For BARCODE ChIP-seq library we used the BARCODE adaptor for generating the library (BARCODE adaptor: PMID 19159457).

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

28067794

Reads aligned (%)

97.7

Duplicates removed (%)

3.6

Number of peaks

1396 (qval < 1E-05)

hg19

Number of total reads

28067794

Reads aligned (%)

96.1

Duplicates removed (%)

6.3

Number of peaks

1390 (qval < 1E-05)