Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
THP-1 RAD21 ChIPseq
cell line
THP-1
cell type
monocytic cell
genotype
MLL-AF9
chip antibody
RAD21 : ab992 Abcam Lot GR12688

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Protein-DNA complexes were immunoprecipitated from sonicated chromatin extract by the specific antibody. ChIP-seq DNA sample prep kit (Illumina, catalog number 1003473) according to the manufacturer’s protocol. For BARCODE ChIP-seq library we used the BARCODE adaptor for generating the library (BARCODE adaptor: PMID 19159457).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
36228709
Reads aligned (%)
50.8
Duplicates removed (%)
9.8
Number of peaks
24017 (qval < 1E-05)

hg38

Number of total reads
36228709
Reads aligned (%)
52.0
Duplicates removed (%)
9.2
Number of peaks
24361 (qval < 1E-05)

Base call quality data from DBCLS SRA