Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

THP-1 CTCF ChIPseq

cell line

THP-1

cell type

monocytic cell

genotype

MLL-AF9

chip antibody

CTCF : 07-729 Millipore Lot. DAM1745366

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Protein-DNA complexes were immunoprecipitated from sonicated chromatin extract by the specific antibody. ChIP-seq DNA sample prep kit (Illumina, catalog number 1003473) according to the manufacturer’s protocol. For BARCODE ChIP-seq library we used the BARCODE adaptor for generating the library (BARCODE adaptor: PMID 19159457).

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

40485849

Reads aligned (%)

96.9

Duplicates removed (%)

11.8

Number of peaks

29084 (qval < 1E-05)

hg19

Number of total reads

40485849

Reads aligned (%)

96.0

Duplicates removed (%)

13.3

Number of peaks

28997 (qval < 1E-05)