Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
WDR5

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
prostate adenocarcinoma
cell line
LNCaP cells
treatment
EtOH
antibody
WDR5, Bethyl Labs (A302-429A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
LNCaP cells were cultured for 3 h in the presence of 10 nM R1881 or vehicle control as indicated and cross-linked by the addition of 1/10 volume of freshly-prepared formaldehyde cross-linking buffer (11% formaldehyde, 10 mM HEPES-KOH [pH 7.6], 100mM NaCl, 1 mM EDTA, 0.5 mM EGTA) to cell culture dishes and incubated for 10 min at room temperature.Glycine was added to a final concentration of 125 mM and cells were incubated for 5 min at room temperature. The samples were sonicated and the sonicated lysates were subjected to immunoprecipitation using the indicated antibodies and protein G Dynabeads. Precipitates were washed and DNA was extracted. KAPA Library Prep Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
18340169
Reads aligned (%)
67.0
Duplicates removed (%)
31.0
Number of peaks
839 (qval < 1E-05)

hg38

Number of total reads
18340169
Reads aligned (%)
69.0
Duplicates removed (%)
29.5
Number of peaks
774 (qval < 1E-05)

Base call quality data from DBCLS SRA