Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Tgif1

Cell type

Cell type Class
Blood
Cell type
MLL-AF9 leukemic cell
NA
NA

Attributes by original data submitter

Sample

source_name
c-kit+ bone marrow cells
strain
C57BL/6
cell type
MLL-AF9 transformed cells
feature
Transduced with pMIG-TGIF1-ER
treatment
4-OHT
chip antibody
TGIF (H-172) (Santa Cruz Biotechnology, catalog# sc-9084, lot# K2608)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lystaes were sonicated to an avarge fragment length of 300bp Precipitated DNA was mixed with E. Coli DNA up to 2ng and libraries were prepared using the NEB Next ChIP-seq library prep master mix for Illumina according to manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
45369785
Reads aligned (%)
90.8
Duplicates removed (%)
28.6
Number of peaks
10325 (qval < 1E-05)

mm9

Number of total reads
45369785
Reads aligned (%)
90.6
Duplicates removed (%)
28.6
Number of peaks
10369 (qval < 1E-05)

Base call quality data from DBCLS SRA