Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Embryo

Cell type

5-13h embryos

NA

NA

Sample

source_name

H3K27ac_mixed

tissue

embryo

age

5-13h

tissue subtype

whole embryo

chip antibody

anti-K27ac

chip antibody vendor

Active Motif

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were digested with MNase, solubilized and ChIP performed using the appropriate antibody. Libraries were prepared as described in Bowman et al., 2013.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

25026335

Reads aligned (%)

21.6

Duplicates removed (%)

14.1

Number of peaks

1904 (qval < 1E-05)

dm3

Number of total reads

25026335

Reads aligned (%)

21.8

Duplicates removed (%)

13.2

Number of peaks

1824 (qval < 1E-05)