Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
5-13h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
H3K27me3_mixed
tissue
embryo
age
5-13h
tissue subtype
whole embryo
chip antibody
anti-K27me3
chip antibody vendor
Active Motif

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were digested with MNase, solubilized and ChIP performed using the appropriate antibody. Libraries were prepared as described in Bowman et al., 2013.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
17588405
Reads aligned (%)
85.5
Duplicates removed (%)
44.7
Number of peaks
9026 (qval < 1E-05)

dm6

Number of total reads
17588405
Reads aligned (%)
85.0
Duplicates removed (%)
45.9
Number of peaks
8152 (qval < 1E-05)

Base call quality data from DBCLS SRA