GSM3401070: mES OCT4-FKBP dTAG Input; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Embryonic stem cells
cell type
V6.5 OCT4-FKBP
chip antibody
None
young_id
20180615_7242
system
Stem Cell
treatment
dTAG-47, 100 nM
treatment duration
24h
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked on plate by addition of 10% volume of 11% formaldehyde solution for 15 minutes at room temperature. 50 million cells were used per ChIP reaction. ChIP was performed according to Young lab protocol (Pete Ralh 4.10) and sonication was performed on a QSonica Q700 tip sonicator. Library construction was performed using the Swift Biosciences ChIP kit by the Whitehead Institute Genome Technology Core. Lysates were clarified from sonicated nuclei and target bound fragments were isolated with antibody.