Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BAP1

Cell type

Cell type Class
Pancreas
Cell type
PANC-1
Tissue
Pancreas/Duct
Disease
Epithelioid Carcinoma

Attributes by original data submitter

Sample

source_name
pancreas
cell line
PANC1
cell type
human pancreatic cancer cell line
treatment
10Gy of ionizing irradiation
chip antibody
BAP1 (D1W9B) (ChIP), Cell Signaling Technology, Cat# 78105
input used for data normalization
Input#1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with the indicated antibodies. Libraries were prepared according to NEBNext® Ultra™ II DNA Library Prep Kit for Illumina and libraries were sequenced on the HiSeq4000 platform.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
32658814
Reads aligned (%)
51.1
Duplicates removed (%)
18.7
Number of peaks
802 (qval < 1E-05)

hg38

Number of total reads
32658814
Reads aligned (%)
52.1
Duplicates removed (%)
17.8
Number of peaks
1157 (qval < 1E-05)

Base call quality data from DBCLS SRA