Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pancreas

Cell type

Pancreas

MeSH Description

A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.

Sample

source_name

pancreas

treatment

Input#1 (Control)

chip antibody

Beads-IgG control

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with the indicated antibodies. Libraries were prepared according to NEBNext® Ultra™ II DNA Library Prep Kit for Illumina and libraries were sequenced on the HiSeq4000 platform.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

32876912

Reads aligned (%)

73.7

Duplicates removed (%)

16.5

Number of peaks

740 (qval < 1E-05)

hg19

Number of total reads

32876912

Reads aligned (%)

72.8

Duplicates removed (%)

17.4

Number of peaks

421 (qval < 1E-05)