Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TERF2

Cell type

Cell type Class
Blood
Cell type
Lymphoblastoid cell line
Tissue
blood
Lineage
mesoderm
Description
parental cell type to lymphoblastoid cell lines

Attributes by original data submitter

Sample

source_name
LCL
chip antibody
TERF2
cell type
LCL

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions. Libraries were prepared according to Illumina's ChIPSeq Sample Prep. protocol (IP-102-1001). Briefly, DNA was end-repaired to produce blunt ends, adenylated for 'A' base addition to the 3' end and then adapter ligated. The adapter ligated DNA was size-selected on an Agarose gel and purified. The purified DNA was enriched by 18 cycles of PCR yielding library fragments of ~290 (insert plus adaptor sequence). The libraries were subjected to hybridisation onto the flowcell for cluster generation and finally sequenced on the Genome Analyzer IIx following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
48523584
Reads aligned (%)
51.7
Duplicates removed (%)
46.7
Number of peaks
1240 (qval < 1E-05)

hg38

Number of total reads
48523584
Reads aligned (%)
53.8
Duplicates removed (%)
45.1
Number of peaks
1194 (qval < 1E-05)

Base call quality data from DBCLS SRA