GSM3396526: G401 BRD9KO H3K4me3 rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Kidney
Cell type
G-401
Primary Tissue
Kidney
Tissue Diagnosis
Wilms' tumor
Attributes by original data submitter
Sample
source_name
ChIPseq_BRD9KO_G401
cell line
G401
cell type
kidney rhabdoid tumor derived cell line
genotype/variation
BRD9KO
chip antibody
H3K4me3 (Cell Signaling Technology: 9751)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was fragmented the adaptive focused acoustics (AFA) technology developed by Covaris for the cell lines. 5-10ng of ChIP DNA is used for library prep using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®