GSM1328459: MC3T3-E1 cells Input DNA (for ChIP-Seq); Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Bone
Cell type
MC3T3-E1
NA
NA
Attributes by original data submitter
Sample
source_name
matrix-depositing MC3T3-E1 subclone 4 cells
cell line
MC3T3-E1
time
day9
cell type
matrix-depositing osteoblast
passages
28-31
strain
C57BL/6
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, nuclear lysates were sonicated and Ctcf-DNA complexes were isolated with Ctcf antibody (Millipore, 07-729). Libraries of purified DNA were generated using Illumina SR adapters (Illumina) following manufacturer’s manual, and were selected for the inserted fragments of 200 ± 50 bp, and sequenced 36 bases on an Illumina Genome Analyzer II.