Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCL11A

Cell type

Cell type Class
Blood
Cell type
CAL-1
NA
NA

Attributes by original data submitter

Sample

source_name
CAL-1 plasmacytoid dendritic cells
cell type
plasmacytoid dendritic cell
chip antibody
Anti-BCL11a polyclonal (Bethyl Labs BL1797)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed by cross-linking proteins to DNA using 1% formaldehyde solution. Immunoprecipitated DNA was sequenced using Illumina sequencers. Pull-downed DNA was applied for generation of sequencing libraries using the NEB ChIP-seq library prep. kit (E6200L ) following by manufacturer’s instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
14508905
Reads aligned (%)
85.5
Duplicates removed (%)
8.0
Number of peaks
500 (qval < 1E-05)

hg19

Number of total reads
14508905
Reads aligned (%)
84.7
Duplicates removed (%)
8.4
Number of peaks
475 (qval < 1E-05)

Base call quality data from DBCLS SRA