Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
Mouse embryonic fibroblasts
cell type
Mouse embryonic fibroblasts
passage
Proliferating passage 4
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as previously described (Thillainadesan et al. 2008) using an antibody for CAP-D3. Ths library was built using the NEB ChIP-Seq Library Prep kit for the Illumina platform. Through the use of Illumina v3 chemistry, sequencing of the library was then performed on a lane of an Illumina HiSeq 2500 instrument using the multiplex paired end protocol (2x100-bases).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
19235388
Reads aligned (%)
98.9
Duplicates removed (%)
17.4
Number of peaks
165 (qval < 1E-05)

mm9

Number of total reads
19235388
Reads aligned (%)
98.8
Duplicates removed (%)
17.5
Number of peaks
131 (qval < 1E-05)

Base call quality data from DBCLS SRA