Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RBM25

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC (CCL-243)
cell line
K562
cell type
Hepatocellular carcinoma
chip antibody
RBM25 (Bethyl Cat# A301-068A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were isolated and sonicated and lysates were cleared-up and applied for purification of RBP-DNA complexes with antibodies. Libraries were constructed according to the instruction of the Illumina Preparing Samples for ChIP Sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
61981843
Reads aligned (%)
64.9
Duplicates removed (%)
19.9
Number of peaks
72675 (qval < 1E-05)

hg38

Number of total reads
61981843
Reads aligned (%)
65.9
Duplicates removed (%)
19.2
Number of peaks
72761 (qval < 1E-05)

Base call quality data from DBCLS SRA