Antigen

Antigen Class

TFs and others

Antigen

RBM22

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

biomaterial_provider

ATCC (CCL-243)

cell line

K562

cell type

Hepatocellular carcinoma

chip antibody

RBM22 (Bethyl Cat# A303-923A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were isolated and sonicated and lysates were cleared-up and applied for purification of RBP-DNA complexes with antibodies. Libraries were constructed according to the instruction of the Illumina Preparing Samples for ChIP Sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

77690124

Reads aligned (%)

70.7

Duplicates removed (%)

36.5

Number of peaks

13439 (qval < 1E-05)

hg19

Number of total reads

77690124

Reads aligned (%)

70.2

Duplicates removed (%)

37.4

Number of peaks

13314 (qval < 1E-05)