GSM3392921: Inhi noAB (rep. 1); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
AS CDK12 HCT116 cells
treatment
5 uM 3-MB-PP1
chip antibody
no antibody
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed with RNAPII, P-Ser2 and P-Ser5 antibodies. Immunoprecipitated DNA was dissolved in H2O and DNA concentration was measured by Qubit fluorometer (Thermo Fisher Scientific) and 4 ng of immunoprecipitated DNA was used for library preparation. ChIP-seq libraries were generated using the KAPA Biosystems Hyper Prep Kit (KK8502) with KAPA Pure Beads (KK8001), and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 and Set 2 (NEB, E7335S, E7500S) with 13x PCR cycles for library amplification, as per manufacturer's instructions. Libraries were run on the fragment analyser (Advanced Analytical Technologies) to check the quality and were sequenced with 50 bp single-end reads on 2 lanes of an Illumina HiSeq 2500 (VBCF Vienna).