Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Macrophages

MeSH Description

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Sample

source_name

Macrophage-control

treatment

Control buffer

gender

male

cell type

Macrophage

chip antibody

rabbit polyclonal anti-H3K27ac (ab4729, Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

H3K27ac enriched genomic DNA and input control DNA (no antibody) was extracted using the Invitrogen Magnify Kit according to the manufacturer's instructions (Invitrogen, Carlsbad, CA) with rabbit polyclonal anti-H3K27ac (ab4729, Abcam, Cambridge, MA). Libraries were generated from gel-purified ~200bp DNA fragments. After adapter ligation and PCR-based amplification, samples were multiplexed into one lane of the Illumina HiSeq platform to generate paired end 50bp reads (Illumina, San Diego, CA).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

39091354

Reads aligned (%)

83.2

Duplicates removed (%)

5.7

Number of peaks

45827 (qval < 1E-05)

hg19

Number of total reads

39091354

Reads aligned (%)

83.0

Duplicates removed (%)

5.8

Number of peaks

45633 (qval < 1E-05)