GSM1326220: Control IgG, replicate2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Adipocyte
Cell type
Adipose stromal cell
NA
NA
Attributes by original data submitter
Sample
source_name
hASC_Control_IgG
cell type
Adipose stromal cell (ASC) adipocyte
chip antibody
rabbit IgG
chip antibody vendor
Santa Cruz Biotechnology
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were harvested and nuclear fractions were enriched by a brief sonication in lysis buffer . Then protein-DNA complexes were isolated with antibody. ChIP-sequencing libraries were prepared using NEBnext chip-seq library Prep master mix set (New England Biolabs, USA) from 5 ng of anti-EBF1 and anti-IgG ChIP DNA according to manufacturer's protocol.The concentration of the library was determined by Nanodrop spectrophotometer (Thermo Fisher Scientific Inc.) and 10 pmol of the DNA was applied for one flow-cell lane.samples were indexed with DNA barcodes and 3 different samples were multiplexed in one library and sequenced using Illumina HiSeq 2000 (single 36 bp reads). Barcode for IgG sample is ATTT and for EBF1 sample is GCCT in replicate 1 In replicate2, barcode for IgG sample is ACAT and for EBF1 sample is TAAT .