GSM3389600: A673 WT input A350C18; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Bone
Cell type
A-673
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
Rhabdomyosarcoma
Attributes by original data submitter
Sample
source_name
A673 Ewing sarcoma cells
cell line
A673
cell type
Ewing sarcoma cells
chip-antibody
none (input DNA)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA-protein cross-linking was done in presence of 1% of paraformaldehyde on 12 × 10^6 cells for each condition for 10 minutes. Cell lysis, chromatin shearing, immunoprecipitation and DNA purification was performed with reagents from iDeal ChIP-seq kit for Transcription Factors (Diagenode, ref: C01010054). Chromatin shearing was carried out in a Bioruptor (Diagenode) using 20 cycles of sonication (30sec high, 30sec off) in TPX tubes (Diagenode, ref: 50001). For immunoprecipitation of MYBL2, 2 µg of a monoclonal ChIP-grade rabbit anti-MYBL2 antibody (Abcam, ref: 76009) were used. IgG and CTCF ChIP was included as negative and positive control, respectively. MYBL2 ChIP and input were sequenced on a HiSeq2500 instrument (Illumina) using 100bp single-end sequencing.