Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYBL2

Cell type

Cell type Class
Bone
Cell type
A-673
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
Rhabdomyosarcoma

Attributes by original data submitter

Sample

source_name
A673 Ewing sarcoma cells
cell line
A673
cell type
Ewing sarcoma cells
chip-antibody
monoclonal ChIP-grade rabbit anti-MYBL2 antibody (Abcam, ref: 76009)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA-protein cross-linking was done in presence of 1% of paraformaldehyde on 12 × 10^6 cells for each condition for 10 minutes. Cell lysis, chromatin shearing, immunoprecipitation and DNA purification was performed with reagents from iDeal ChIP-seq kit for Transcription Factors (Diagenode, ref: C01010054). Chromatin shearing was carried out in a Bioruptor (Diagenode) using 20 cycles of sonication (30sec high, 30sec off) in TPX tubes (Diagenode, ref: 50001). For immunoprecipitation of MYBL2, 2 µg of a monoclonal ChIP-grade rabbit anti-MYBL2 antibody (Abcam, ref: 76009) were used. IgG and CTCF ChIP was included as negative and positive control, respectively. MYBL2 ChIP and input were sequenced on a HiSeq2500 instrument (Illumina) using 100bp single-end sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
99306793
Reads aligned (%)
68.9
Duplicates removed (%)
28.4
Number of peaks
12435 (qval < 1E-05)

hg38

Number of total reads
99306793
Reads aligned (%)
70.8
Duplicates removed (%)
27.3
Number of peaks
12892 (qval < 1E-05)

Base call quality data from DBCLS SRA