Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD3

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549 DMSO
cell line
A549
agent
DMSO
chip antibody
BRD3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and DNA fragments were isolated with antibody. Libraries were prepared according to VAHTS Universal DNA Library Prep Kit( ND604). Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment(3' to 5' exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3' end. After adapter ligation DNA was PCR amplified with Illumina primers for 10 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina Hiseq 2500 following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
6641405
Reads aligned (%)
80.3
Duplicates removed (%)
7.2
Number of peaks
86 (qval < 1E-05)

hg19

Number of total reads
6641405
Reads aligned (%)
79.5
Duplicates removed (%)
8.0
Number of peaks
65 (qval < 1E-05)

Base call quality data from DBCLS SRA