Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Adult
Cell type
Adult head
NA
NA

Attributes by original data submitter

Sample

source_name
Drosophila head
antibody for chip
H3K9me2
age
adult (1-2 weeks)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The homogenized and cleared fly heads were sonicated to a length between 200~500 bp. The sheared chromatin was precleared with Protein A beads then incubated with 5ml of antibody and 30ml of Protein A beads at 4°C for 3 hours. After thorough washing, the samples were eluted by 10% SDS and Protease K at 65°C for 8 hours. The eluted chromatin was extracted by phenol-chloroform clean-up followed by ethanol precipitation. RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
16480041
Reads aligned (%)
60.1
Duplicates removed (%)
36.1
Number of peaks
2283 (qval < 1E-05)

dm3

Number of total reads
16480041
Reads aligned (%)
60.7
Duplicates removed (%)
34.7
Number of peaks
1692 (qval < 1E-05)

Base call quality data from DBCLS SRA