GSM3383358: conventional CD4 T cell Tcf1 ChIP-Seq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Tcf7
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
conventional CD4+ T cells
genotype
wild type
tissue
effector CD4 T cells
cell phenotype
TCRb+, CD4+, CD25-, Foxp3-Cre-GFP-
antibody
anti-Tcf1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Conventional or regulatory CD4+ T cells were sort-purified from WT Foxp3-cre-GFP mice, and the Tcf1-deficient CD4+ T cells were sorted from CD4-Cre+ Tcf1 fl/fl mice. The cells were then fixed and sonicated to generate chromatin fragments, followed by immunoprecipitation with antiserum against Tcf1, which were then properly washed and immunoprecipitated DNA extracted. The DNA segments from ChIP were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.