Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

MEC-2

Primary Tissue

Blood

Tissue Diagnosis

Leukemia

Sample

source_name

MEC2

cell type

CLL cell line

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and samples taken before immunoprecipitation. Immunoprecipitated genomic DNA was prepared for Illumina sequencing using the ThruPLEX DNA-seq kit (Rubicon Genomics).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

153758251

Reads aligned (%)

95.7

Duplicates removed (%)

5.5

Number of peaks

2283 (qval < 1E-05)

hg19

Number of total reads

153758251

Reads aligned (%)

95.0

Duplicates removed (%)

6.5

Number of peaks

1776 (qval < 1E-05)