Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
CLL
Tissue
blood
Lineage
mesoderm
Description
chronic lymphocytic leukemia cell, T-cell lymphocyte

Attributes by original data submitter

Sample

source_name
CII
cell type
CLL cell line
chip antibody
H3K27ac abcam ab4729

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Immunoprecipitated genomic DNA was prepared for Illumina sequencing using the ThruPLEX DNA-seq kit (Rubicon Genomics).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
57497399
Reads aligned (%)
98.3
Duplicates removed (%)
4.0
Number of peaks
2016 (qval < 1E-05)

hg19

Number of total reads
57497399
Reads aligned (%)
97.4
Duplicates removed (%)
5.5
Number of peaks
1348 (qval < 1E-05)

Base call quality data from DBCLS SRA