Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

MEC-2

Primary Tissue

Blood

Tissue Diagnosis

Leukemia

Sample

source_name

MEC2

cell type

CLL cell line

chip antibody

H3K27ac abcam ab4729

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Immunoprecipitated genomic DNA was prepared for Illumina sequencing using the ThruPLEX DNA-seq kit (Rubicon Genomics).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

33399752

Reads aligned (%)

96.8

Duplicates removed (%)

8.1

Number of peaks

46469 (qval < 1E-05)

hg19

Number of total reads

33399752

Reads aligned (%)

96.5

Duplicates removed (%)

8.5

Number of peaks

48041 (qval < 1E-05)