Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
eralpha_Control_E2+4OHT
cell line
MCF-7
cell type
breast cancer cell line
treatment
Control-E2+4OHT
chip antibody
ER (CST:D8H8)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation was performed with a SimpleChIP Enzymatic Chromatin IP kit (CST) according to the manufacturer's instructions. Sequencing libraries were prepared with 1.8 ng of the precipitated DNAs using an Ion Xpress Plus Fragment Library kit (Thermo Fisher Scientific) according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
37977632
Reads aligned (%)
76.0
Duplicates removed (%)
19.9
Number of peaks
10309 (qval < 1E-05)

hg38

Number of total reads
37977632
Reads aligned (%)
78.4
Duplicates removed (%)
17.9
Number of peaks
11571 (qval < 1E-05)

Base call quality data from DBCLS SRA