Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Uterus
Cell type
Endometrial stromal cells
NA
NA

Attributes by original data submitter

Sample

source_name
Human endometrial stromal cells (hESC)
disease state
Polycystic ovary syndrome
cell type
endometrial stromal cells
antibody
anti-AR antibody
treatment
DHT

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and AR-DNA complexes were isolated with antibody as per manufacturers instructions (Chromatrap® Pro-A kit). Isolated DNA was sent to Active Motif® for library construction. AM prepared the libraries according to Illumina's instructions accompanying the True-Seq ChIP Library Preparation Kit (IP-202-1012, Illumina®).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
34882008
Reads aligned (%)
42.8
Duplicates removed (%)
53.8
Number of peaks
2381 (qval < 1E-05)

hg38

Number of total reads
34882008
Reads aligned (%)
44.4
Duplicates removed (%)
52.5
Number of peaks
2329 (qval < 1E-05)

Base call quality data from DBCLS SRA