Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ELK1

Cell type

Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA

Attributes by original data submitter

Sample

source_name
Primary human dermal fibroblast
tissue
human dermal skin
passages
5
chip-antibody
Anti-ELK1 antibody[E277] (abcam,ab32106)
condition
control

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and ELK1-DNA complexes were isolated with Anti-ELK1 antibody[E277] - ChIP Grade (abcam,ab32106) DNA samples were end-repaired, A tailed, and adaptor ligated using TruSeq Nano DNA Sample Prep Kit (#FC-121-4002, Illumina), following the manufacturer's instructions. ~200-1500 bp fragments were size selected using AMPure XP beads. The final size of the library was confirmed by Agilent 2100 Bioanalyzer.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg19

Number of total reads
30605366
Reads aligned (%)
125.3
Duplicates removed (%)
29.7
Number of peaks
377 (qval < 1E-05)

hg38

Number of total reads
30605366
Reads aligned (%)
127.3
Duplicates removed (%)
29.3
Number of peaks
850 (qval < 1E-05)

Base call quality data from DBCLS SRA