Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
REST

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
REST, LNCaP cells
cell line
LNCaP
cell type
Androgen sensitive prostate cancer cells
chip antibody
anti-REST (17-641, Millipore)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and REST-DNA complexes were isolated with antibody. Libraries were prepared following standard Illumina´s protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
16331808
Reads aligned (%)
90.8
Duplicates removed (%)
3.6
Number of peaks
2986 (qval < 1E-05)

hg38

Number of total reads
16331808
Reads aligned (%)
92.3
Duplicates removed (%)
2.6
Number of peaks
2970 (qval < 1E-05)

Base call quality data from DBCLS SRA