Antigen

Antigen Class

TFs and others

Antigen

REST

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

REST, LNCaP cells

cell line

LNCaP

cell type

Androgen sensitive prostate cancer cells

chip antibody

anti-REST (17-641, Millipore)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and REST-DNA complexes were isolated with antibody. Libraries were prepared following standard Illumina´s protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

17142413

Reads aligned (%)

96.6

Duplicates removed (%)

1.7

Number of peaks

2219 (qval < 1E-05)

hg19

Number of total reads

17142413

Reads aligned (%)

95.8

Duplicates removed (%)

2.6

Number of peaks

2222 (qval < 1E-05)