Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GFP

Cell type

Cell type Class
Adult
Cell type
Whole worm
NA
NA

Attributes by original data submitter

Sample

source_name
whole animal
tissue
whole animal
genotype
pmk-1(km25);atf-7(qd328[atf-7::2xTY1::GFP])
bacterial exposure
OP50
exposure length
4 hours
age
L4
antibody
Ab290 (ChIP-grade polyclonal antibody from Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After three washes in M9 buffer, animal pellets were resuspended in an equal volume of PBS + complete ULTRA protease inhibitor tablets (Roche) and flash frozen in liquid nitrogen and stored at -80°C until chromatin immunoprecipitation (ChIP). ChIP was preformed using Abcam antibody Ab290. Libraries were prepared using the SPRIworks Fragment Library System (Beckman Coulter) and single-end sequenced on an Illumina HiSeq2000 sequencer with v3 chemistry in high-output mode.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

ce11

Number of total reads
15303144
Reads aligned (%)
56.8
Duplicates removed (%)
62.1
Number of peaks
889 (qval < 1E-05)

ce10

Number of total reads
15303144
Reads aligned (%)
56.8
Duplicates removed (%)
62.1
Number of peaks
889 (qval < 1E-05)

Base call quality data from DBCLS SRA