Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Neural
Cell type
Glioblastoma
MeSH Description
A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.

Attributes by original data submitter

Sample

source_name
Patient Derived Glioblastoma Stem Cell
antibody
H3K4me3
cell treatment
No treatment

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA extraction protocol is described in the method section For ChIP-seq and N6-mA DIPseq, NEB NEBNext® Ultra™ DNA Library Prep Kit

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
78689611
Reads aligned (%)
75.9
Duplicates removed (%)
6.9
Number of peaks
21701 (qval < 1E-05)

hg19

Number of total reads
78689611
Reads aligned (%)
75.5
Duplicates removed (%)
7.0
Number of peaks
21141 (qval < 1E-05)

Base call quality data from DBCLS SRA