GSM3356423: INPUT 22Rv1 delSite1 Clone11/12-F6 rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Prostate
Cell type
22Rv1
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
22Rv1 cells
cell line
prostate carcinoma cell line 22Rv1
genotype
Site 1 complete deletion (chr1:154850252-154851859 in hg19)
grna used
11, 12
clone number
F6
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatins were prepared using the protocol previously published (PMID: 29429977). Briefly, cells were crosslinked by 1%formedelhyde for 10 mins and lysised into nuclei. Nuclei were sonicated using BioRuptor to break into chromatin fragments. Libraries were prepared using KAPA Hyper Prep Kit (Kapa #KK8505) according to provided protocol