Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Prostate

Cell type

22Rv1

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

22Rv1 cells

cell line

prostate carcinoma cell line 22Rv1

genotype

Site 1 complete deletion (chr1:154850252-154851859 in hg19)

grna used

11, 12

clone number

D8

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatins were prepared using the protocol previously published (PMID: 29429977). Briefly, cells were crosslinked by 1%formedelhyde for 10 mins and lysised into nuclei. Nuclei were sonicated using BioRuptor to break into chromatin fragments. Libraries were prepared using KAPA Hyper Prep Kit (Kapa #KK8505) according to provided protocol

Sequencing Platform

instrument_model

Illumina HiSeq 3000

hg38

Number of total reads

44611122

Reads aligned (%)

99.0

Duplicates removed (%)

7.9

Number of peaks

1128 (qval < 1E-05)

hg19

Number of total reads

44611122

Reads aligned (%)

98.1

Duplicates removed (%)

9.6

Number of peaks

1129 (qval < 1E-05)