GSM3356671: THP1 pooled input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
THP1_pooled_input
cell line
THP1
cell type
Monocytic/Innate Immune
passages
12
chip antibody
RNA Pol II antibody (mAb clone ab5095,Abcam)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The pellets were sonicated and the chromatin was treated with Rnase, proteinase K and heat (65oC), followed by phenol and chlorophorm extractions, and ethanol precipitation. Pellets were resuspended in 10mM Tris, 1mM EDTA, and quantified in Nanodrop. Libraries were prepared using Illumina Hi-Seq, and mapped to the genome using BWA algorithm using default settings.