Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPSC derived cardiac cells
NA
NA

Attributes by original data submitter

Sample

source_name
iPSC-CMs
cell type
iPSC-CMs
day post-differentiation
day 40
genotype/variation
LMNA WT/MT
chip antibody
H3K4me3 (Active Motif 39159)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and LMNA-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using The Ion Proton™ System protocols.

Sequencing Platform

instrument_model
NextSeq 550

hg38

Number of total reads
59207590
Reads aligned (%)
81.3
Duplicates removed (%)
16.2
Number of peaks
22234 (qval < 1E-05)

hg19

Number of total reads
59207590
Reads aligned (%)
81.0
Duplicates removed (%)
16.3
Number of peaks
21845 (qval < 1E-05)

Base call quality data from DBCLS SRA