Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EHMT2

Cell type

Cell type Class
Muscle
Cell type
RH-41
Primary Tissue
Skelatal Muscle
Tissue Diagnosis
Rhabdomyosarcoma

Attributes by original data submitter

Sample

source_name
ARMS Lung metastasis 7-year-old female
cell type
Alveolar rhabdomyosarcoma metastatic cell line
passages
20-22
cell line
RH41
chip antibody
G9a abcam (#40452)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed with Farnham lysis buffer followed by RIPA buffer, Chromatin sheared and DNA was extracted as per manufacturer's protocol ChIP kit (#17-295, Mercmillipore, Bedford, MA, USA). 30ng ChIPed DNA and corresponding input were used for ChIP-seq library construction (New England Biolabs). Library fragment size was determined using the DNA 1000 Kit on the Agilent Bioanalyzer (Agilent Technologies). Libraries were pooled in equimolar and cluster generation was performed on the Illumina cBOT system. Sequencing (150bp pair-end) was performed on the Illumina MiSeq according to manufacturer's protocol at the Duke-NUS Genome Biology Facility, Singapore. Sequencing reads were mapped against hg19 and G9a bound regions were identified by MACS2.

Sequencing Platform

instrument_model
Illumina MiSeq

hg19

Number of total reads
8866487
Reads aligned (%)
145.0
Duplicates removed (%)
17.8
Number of peaks
17494 (qval < 1E-05)

hg38

Number of total reads
8866487
Reads aligned (%)
145.6
Duplicates removed (%)
17.7
Number of peaks
17512 (qval < 1E-05)

Base call quality data from DBCLS SRA