Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

HEK293

kdm inhibitor

KDOPZ-36

cell line

HEK293

chip antibody

none (input)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Library was constructed using the NEB Ulra II DNA library prep kit

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

25605661

Reads aligned (%)

94.8

Duplicates removed (%)

2.3

Number of peaks

477 (qval < 1E-05)

hg19

Number of total reads

25605661

Reads aligned (%)

94.1

Duplicates removed (%)

2.5

Number of peaks

418 (qval < 1E-05)