Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

HEK293

kdm inhibitor

KDOPZ-25

cell line

HEK293

chip antibody

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Library was constructed using the NEB Ulra II DNA library prep kit

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

23236103

Reads aligned (%)

96.2

Duplicates removed (%)

2.6

Number of peaks

2621 (qval < 1E-05)

hg19

Number of total reads

23236103

Reads aligned (%)

95.4

Duplicates removed (%)

2.7

Number of peaks

2546 (qval < 1E-05)