Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Dendritic Cells
MeSH Description
ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Attributes by original data submitter

Sample

source_name
Input DNA
strain
CBA/J
Sex
female
tissue
Lung
cell type
CD11c+ dendritic cells
age
6-8 weeks
condition
Day 14 post intratracheal Cryptococcus neoformans 10^4 CFU
treatment
anti-TNFalpha antibody 14 days prior to cell isolation
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
According to previously published ultra-low input native ChIP-seq protocol (Brind'Amour J, Liu S, Hudson M, Chen C, Karimi MM, Lorincz MC. An ultra-low-input native ChIP-seq protocol for genome wide profiling of rare cell populations. Nature Communications, 2015; 6:6033).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
53703898
Reads aligned (%)
97.3
Duplicates removed (%)
16.9
Number of peaks
511 (qval < 1E-05)

mm9

Number of total reads
53703898
Reads aligned (%)
97.0
Duplicates removed (%)
16.8
Number of peaks
555 (qval < 1E-05)

Base call quality data from DBCLS SRA