Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Gonad

Cell type

HEYA8

NA

NA

Sample

source_name

Ovarian cancer cell line

cell line

HEYA8

chip antibody

H3K4me3 (CS-003-100, Diagenode)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed from the ChIP-DNA using Illumina TruSeq ChIP Sample Prep kit (IP-202-1012), with 15 to 18 cycles of PCR amplification. DNA size selection was performed using AMPure XP beads.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

57825334

Reads aligned (%)

97.9

Duplicates removed (%)

31.4

Number of peaks

46664 (qval < 1E-05)

hg19

Number of total reads

57825334

Reads aligned (%)

97.1

Duplicates removed (%)

33.4

Number of peaks

45897 (qval < 1E-05)