GSM3328065: SKOV3 with H3K27me3 ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Gonad
Cell type
SK-OV-3
Primary Tissue
Ovary
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Ovarian cancer cell line
cell line
SKOV3
chip antibody
H3K27me3 (07-449, Millipore)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed from the ChIP-DNA using Illumina TruSeq ChIP Sample Prep kit (IP-202-1012), with 15 to 18 cycles of PCR amplification. DNA size selection was performed using AMPure XP beads.