Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Gonad

Cell type

SK-OV-3

Primary Tissue

Ovary

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

Ovarian cancer cell line

cell line

SKOV3

chip antibody

H3K27ac (ab4729, Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed from the ChIP-DNA using Illumina TruSeq ChIP Sample Prep kit (IP-202-1012), with 15 to 18 cycles of PCR amplification. DNA size selection was performed using AMPure XP beads.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

45283022

Reads aligned (%)

98.7

Duplicates removed (%)

23.3

Number of peaks

13447 (qval < 1E-05)

hg19

Number of total reads

45283022

Reads aligned (%)

98.4

Duplicates removed (%)

23.8

Number of peaks

13407 (qval < 1E-05)