Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

PEO1

Primary Tissue

Ovary

Tissue Diagnosis

Carcinoma

Sample

source_name

Ovarian cancer cell line

cell line

PEO1

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed from the ChIP-DNA using Illumina TruSeq ChIP Sample Prep kit (IP-202-1012), with 15 to 18 cycles of PCR amplification. DNA size selection was performed using AMPure XP beads.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

59179834

Reads aligned (%)

98.3

Duplicates removed (%)

8.7

Number of peaks

1713 (qval < 1E-05)

hg19

Number of total reads

59179834

Reads aligned (%)

97.5

Duplicates removed (%)

10.5

Number of peaks

1349 (qval < 1E-05)