Antigen

Antigen Class

TFs and others

Antigen

BRD9

Cell type

Cell type Class

Others

Cell type

Aska

NA

NA

Sample

source_name

Aska (SS)

cell line

Aska

chip antibody

Abcam (ab137245)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed using standard protocols (Millipore, Billerica, MA). Specifically, cells were fixed in 1% formaldehyde (Sigma Aldrich, F8775) for 10min at 37C and quenched with 125mM glycine for 5min at 37C. After washing, nuclei were sonicated using Covaris Sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-sequencing libraries were prepared with the Rubicon Thruplex DNAseq prep kit using standard protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

45980542

Reads aligned (%)

96.3

Duplicates removed (%)

20.3

Number of peaks

16657 (qval < 1E-05)

hg19

Number of total reads

45980542

Reads aligned (%)

94.2

Duplicates removed (%)

26.0

Number of peaks

17364 (qval < 1E-05)