Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Hmr

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
SL2 cells
tissue
SL2 cells
genotype
pMT - HMR-FLAG-HA
treatment
induced (CuSO4 250 uM)
chip antibody
ChIP anti-HMR

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Drosophila SL2 cells stably transfected with FLAG-HA-HMR under a CuSO4 inducible promoter (pMT) were grown at 26˚C in Schneider Drosophila medium (Invitrogen) supplemented with 10% fetal calf serum and antibiotics (100 units/mL penicillin and 100 μg/mL streptomycin). Transfected cells were selected with 20 ug/mL Hygromycin B. Cells were grown to confluence in 550 mL flasks and induced for 20-24h with 250 uM CuSO4 before harvesting for chromatin immunoprecipitation.Chromatin immunoprecipitation was essentially performed as in Gerland et al., 2017. For each ChIP reaction, chromatin isolated from 1–2 x 106 cells was incubated with rat anti-HMR 2C10 antibody pre-coupled to Protein A/G Sepharose through a rabbit IgG anti-rat. ChIP-seq Library

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
9389970
Reads aligned (%)
83.8
Duplicates removed (%)
9.3
Number of peaks
2607 (qval < 1E-05)

dm6

Number of total reads
9389970
Reads aligned (%)
83.1
Duplicates removed (%)
11.8
Number of peaks
2300 (qval < 1E-05)

Base call quality data from DBCLS SRA