Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
LNCaP-FGC immortalized prostate cancer cell line
agent
5nM DHT, 3uM Enzalutamide
time point
6 hours
chip antibody
BRD4 (Bethyl, Cat A301-985A100, Lot A301-985A50-6)
cell line
LNCaP-FGC prostate adenocarcinoma cancer cell line

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated, fixed cell pellets and protein-DNA complexes were isolated with antibody. Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
34719339
Reads aligned (%)
71.1
Duplicates removed (%)
24.8
Number of peaks
3035 (qval < 1E-05)

hg38

Number of total reads
34719339
Reads aligned (%)
73.2
Duplicates removed (%)
23.2
Number of peaks
2774 (qval < 1E-05)

Base call quality data from DBCLS SRA